Purification of Follicle-stimulating Hormone from Human Pituitary Glands
نویسندگان
چکیده
منابع مشابه
[Purification of follicle-stimulating hormone from human pituitary glands].
A highly purified follicle-stimulating hormone from human pituitary glands has been prepared by ammonium sulfate and ethanol fractionation, gel filtration on Sephadex G-100, ion exchange chromatography on carboxymethyl cellulose, zone electrophoresis on cellulose columns, and preparative continuous flow polyacrylamide gel electrophoresis. The final product has shown lO,OOO-fold purification ove...
متن کاملPurification of Follicle-stimulating Hormone from Human Pituitary Glands*
A highly purified follicle-stimulating hormone from human pituitary glands has been prepared by ammonium sulfate and ethanol fractionation, gel filtration on Sephadex G-100, ion exchange chromatography on carboxymethyl cellulose, zone electrophoresis on cellulose columns, and preparative continuous flow polyacrylamide gel electrophoresis. The final product has shown lO,OOO-fold purification ove...
متن کاملPurification and properties of follicle-stimulating hormone from sheep pituitary glands.
A highly purified follicle-stimulating hormone (FSH) preparation has been obtained from sheep pituitary glands by extraction with ethanol followed by fractionation with metaphosphoric acid and ammonium sulfate, gel filtration on Sephadex G-150, ion exchange chromatography on carboxymethyl Sephadex, hydroxylapatite chromatography, preparative acrylamide disc gel electrophoresis, and gel filtrati...
متن کاملAmino acid sequence of the beta subunit of follicle-stimulating hormone from human pituitary glands.
The beta subunit of follicle-stimulating hormone (FSH-beta) from human pituitary glands was reduced and S-aminoethylated prior to thermolytic, tryptic, and chymotryptic digestions. Each digest was gel-filtered on Sephadex G-50 to seperate the glycopeptides. The glycopeptides and the peptides were isolated by high voltage paper electrophoresis at pH 6, 3.5, and 2.0. The purity of the isolated pe...
متن کاملAmino Acid Sequence of the ,8 Subunit of Follicle-stimulating Hormone from Human Pituitary Glands*
The p subunit of follicle-stimulating hormone (FSH-@) from human pituitary glands was reduced and S-aminoethylated prior to thermolytic, tryptic, and chymotryptic digestions. Each digest was gel-filtered on Sephadex G-50 to separate the glycopeptides from the peptides. The glycopeptides and the peptides were isolated by high voltage paper electrophoresis at pH 6, 3.5, and 2.0. The purity of the...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)95815-1